09-882
Anti-ALKBH3 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... ALKBH3(221120)
General description
ABH3 is a member of the Fe(II)/2OG-dependent dioxygenase superfamily, which couples substrate oxidation by converting 2-oxoglutarate (2OG) into succinate and CO2. In humans, ABH3 plays a critical role in alkylated DNA/RNA damage repair and in cancer therapy. While ABH3 may repair alkylated RNA, it demonstrates greater preference for the repair of single- over double-stranded alkylated DNA through oxidative demethylation of 1-methyladenine and 3-methylcytosine.
Specificity
Other homologies Orangutan 98% and Rhesus Monkey 97% sequence homology.
This antibody recognizes the N-terminus of ALKBH3.
Immunogen
Epitope: N-terminus
Histidine-tagged-recombinant protein corresponding to the the N-terminus of human ALKBH3.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-ALKBH3 Antibody (rabbit polyclonal antibody) validated in WB to detect ALKBH3 also known as Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1.
Western Blot Analysis: A representative lot from an independent laboratory detected ALKBH3 in PC-3 and LNCap whole cell extract. (Dango, S., et al. (2011). Mol Cell. {Epub ahead of print.})
Quality
Evaluated by Western Blot in human prostate tissue lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.
Target description
~35 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human prostate tissue lysate
Human prostate tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zhuojia Chen et al.
Nucleic acids research, 47(5), 2533-2545 (2018-12-13)
Transfer RNA is heavily modified and plays a central role in protein synthesis and cellular functions. Here we demonstrate that ALKBH3 is a 1-methyladenosine (m1A) and 3-methylcytidine (m3C) demethylase of tRNA. ALKBH3 can promote cancer cell proliferation, migration and invasion.
Yuko Ueda et al.
Biochemistry and biophysics reports, 5, 476-481 (2016-02-10)
Human AlkB homolog 3 (ALKBH3), a homolog of the Escherichia coli protein AlkB, demethylates 1-methyladenine and 3-methylcytosine (3-meC) in single-stranded DNA and RNA by oxidative demethylation. Immunohistochemical analyses on clinical cancer specimens and knockdown experiments using RNA interference in vitro
Qi Li et al.
The Journal of biological chemistry, 291(21), 11083-11093 (2016-03-27)
The AlkB repair enzymes, including Escherichia coli AlkB and two human homologues, ALKBH2 and ALKBH3, are iron(II)- and 2-oxoglutarate-dependent dioxygenases that efficiently repair N(1)-methyladenine and N(3)-methylcytosine methylated DNA damages. The development of small molecule inhibitors of these enzymes has seen
Yuko Ueda et al.
Scientific reports, 7, 42271-42271 (2017-02-17)
The mammalian AlkB homolog (ALKBH) family of proteins possess a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. A similar domain in the Escherichia coli AlkB protein catalyzes the oxidative demethylation of 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) in both DNA and RNA. AlkB
Hanqing He et al.
Blood science (Baltimore, Md.), 2(4), 137-143 (2020-10-23)
ALKBH3, a demethylase responsible for demethylating N1-methyladenosine (m1A) in mRNA and N1-methyldeoxyadenosine in single-stranded DNA, plays an important role in DNA repair and cancer cell proliferation. However, its function in hematopoietic stem cells (HSCs) is unknown. In this study, we
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