07-2162

Anti-PCNA Antibody

from rabbit

• Synonym(s): proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_002583
• UniProt accession no.: P12004
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PCNA(5111)

Description

General description

Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family and is comprised of three identical monomers forming a heterodimer. Due to its unique structure, PCNA is able to increase the processivity of DNA polymerase delta by using its outer domains to be involved in protein-protein interaction while still tethered to and freely sliding along the DNA. PCNA is a critical regulatory component in the onset of leading strand DNA replication and the termination of lagging strand synthesis. Studies have also indicated a role for PCNA in the recruitment and regulation of MMR pathway repair proteins. PCNA is also able to interact with a wide variety of cell cycle proteins and serves to coordinate several proteins participating in DNA processes, such as apoptosis.

Specificity

Other homologies: Bovine, rabbit, canine, and panda (99% sequence homology).

Immunogen

GST-tagged recombinant protein corresponding to human PCNA.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.

Western Blot Analysis: 1 µg/mL from a previous lot detected PCNA on 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PCNA in A431 cells.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PCNA on 10 µg of A431 cell lysate.

Target description

~ 35 kDa observed. PCNA has a predicted molecular weight of 29 kDa, however, it can be monoubiquitinated, resulting in a ~ 35 kDa band.

Physical form

Format: Purified

Protein A

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable