07-2051

Anti-PEMT Antibody (CT)

from rabbit

• Synonym(s): phosphatidylethanolamine N-methyltransferase
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_009100.2
• UniProt accession no.: Q9UBM1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PEMT(10400)

Description

General description

PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.

Specificity

Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).

This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.

Immunogen

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.

Application

ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA

Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).

Research Category
Epigenetics & Nuclear Function

Neuroscience

Research Sub Category
Chromatin Biology

This PEMT antibody is validated for use in WB, ICC & ELISA for the detection of the PEMT protein.

Quality

Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).

Target description

~22 kDa

Physical form

Format: Purified

Protein A chromatography

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Analysis Note

Control
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable