07-1533
Anti-JMJD3 (NT) Antibody
from rabbit
Synonym(s):
JMJD, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, jumonji domain containing 3, jumonji domain containing 3, histone lysine demethylase
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KDM6B(23135)
General description
JMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation
Specificity
Not tested in other species.
Recognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products.
Immunogen
Epitope: N-terminus
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3.
Application
ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA.
Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.
Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).
Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.
This antibody is reported by an
outside laboratory to be suitable for ELISA.
Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.
Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).
Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Chromatin Biology
Histone Modifying Proteins
Chromatin Biology
Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3.
Quality
Immunocytochemisty:
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
Target description
~180 kDa
Physical form
Format: Purified
Protein G Chromatography
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cells
HeLa cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chen Zhang et al.
International journal of molecular sciences, 24(13) (2023-07-14)
Stem cells from the apical papilla (SCAPs) are used to regulate the microenvironment of nerve defects. KDM6B, which functions as an H3K27me3 demethylase, is known to play a crucial role in neurogenesis. However, the mechanism by which KDM6B influences the
Jessica M Huszar et al.
FEBS letters, 588(9), 1850-1856 (2014-04-15)
Chromatin remodeling is important for cell differentiation. Histone methyltransferase EZH2 and histone demethylase JMJD3 (KDM6B) modulate levels of histone H3 lysine 27 trimethylation (H3K27me3). Interplay between the two modulators influence lineage specification in stem cells. Here, we identified microRNA MIR146A
Hongde Li et al.
Nature cell biology, 24(3), 373-383 (2022-02-19)
Metabolic reprogramming is central to oncogene-induced tumorigenesis by providing the necessary building blocks and energy sources, but how oncogenic signalling controls metabolites that play regulatory roles in driving cell proliferation and tumour growth is less understood. Here we show that
K Almstrup et al.
British journal of cancer, 103(8), 1269-1276 (2010-09-09)
The majority of testicular germ cell cancers develop through a pre-invasive carcinoma in situ (CIS) stage. The CIS cell is a neoplastic counterpart of foetal germ cells. During their development, foetal germ cells undergo extensive and essential epigenetic modifications, but
Shaozhen Feng et al.
The Journal of biological chemistry, 298(5), 101816-101816 (2022-03-13)
Jumonji domain-containing protein-3 (JMJD3), a histone H3 lysine 27 (H3K27) demethylase, promotes endothelial regeneration, but its function in neointimal hyperplasia (NIH) of arteriovenous fistulas (AVFs) has not been explored. In this study, we examined the contribution of endothelial JMJD3 to
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