06-727

Anti-FcεRI Antibody, γ subunit

Upstate^®, from rabbit

• Synonym(s): High affinity immunoglobulin epsilon receptor subunit gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain, FcR gamma, IgE Fc receptor subunit gamma
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P30273
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FCER1A(2205)

Description

General description

High affinity immunoglobulin epsilon receptor subunit gamma (UniProt P20411; also known as Fc-epsilon RI-gamma, Fc receptor gamma-chain, FceRI gamma, FcR gamma, IgE Fc receptor subunit gamma) is encoded by the Fcer1g (also known as Fce1g) gene (Gene ID: 25441) in rat species. FceR1 gamma is a single-pass type I membrane protein that associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. FceRI gamma is found exclusively on mast cells and basophils. In rodents Fc epsilon RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding alpha subunit, one beta subunit and a dimer of disulfide-linked gamma subunits. When multivalent allergens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. (Ref.: Blank, U., et al. (1989). Nature 337(6203); 187-189).

Specificity

Other species cross-reactivity not tested.

Recognizes and is specific for FcεRI, γ-subunit, MW 10kDa.

Immunogen

KLH-conjugated linear peptide corresponding to 7 amino acids from the C-terminal region of rat FceRI gamma subunit.

Application

Anti-FceRI, gamma subunit, Cat. No. 06-727, is a rabbit polyclonal antibody that detects FceRI, subunit and has been tested for use in Flow Cytometry and Western Blotting.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected FceRI, gamma subunit in rat basophilic leukemia cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by western blot on RIPA lysate of RBL cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.

Target description

30 kDa

Physical form

Format: Purified

Protein A purified

Purified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
RBL cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable