Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen
Linear peptide corresponding to trimethyl Histone H3 (Lys27).
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl Histone H3 (Lys27) in NIH/3T3 cells.
Research Category Epitope Tags & General Use
Research Sub Category Epitope Tags
This Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Alexa Fluor™ 488 Conjugate is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).
Quality
Evaluated by Immunocytochemistry Analysis in HepG2 cells
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HepG2 cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Target description
17 kDa calculated
Physical form
Protein G Purified
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA, and 0.1% Sodium Azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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