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04-282

Sigma-Aldrich

Anti-phospho-EGFR (Thr654) Antibody, clone 3F2

clone 3F2, Upstate®, from mouse

Synonym(s):

ERBB, ERBB1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F2, monoclonal

species reactivity

mouse, human, canine, rat

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

UniProt accession no.

P00533

shipped in

wet ice

target post-translational modification

phosphorylation (pThr654)

Gene Information

dog ... Egfr(404306)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Specificity

Recognizes phosphorylated EGFR on Threonine 654

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR

Application

Detect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.

Quality

Routinely evaluated by immunoblot.

Target description

180 kDa

Physical form

100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Format: Purified

Analysis Note

Control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane
Hunter, T, et al
Nature, 311, 480-483 null
Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
Aifa, Sami, et al
Biochemical and biophysical research communications, 347, 381-387 (2006)
Elena Odintsova et al.
The Journal of biological chemistry, 288(36), 26323-26334 (2013-07-31)
Ligand-induced ubiquitylation of EGF receptor (EGFR) is an important regulatory mechanism that controls endocytic trafficking of the receptor and its signaling potential. Here we report that tetraspanin CD82/KAI1 specifically suppresses ubiquitylation of EGFR after stimulation with heparin-binding EGF or amphiregulin

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