04-1557
Anti-SIRT1 Antibody, clone 10E4
clone 10-E-4, from mouse
Synonym(s):
SIR2-like protein 1, sir2-like 1, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10-E-4, monoclonal
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SIRT1(23411)
General description
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
Specificity
This antibody recognizes SIRT1.
Immunogen
Epitope: Unknown
His-tagged recombinant protein corresponding to human SIRT1.
application
Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting.
Immunoprecipitation: Reported by independent researcher using representative lot.
Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
Immunohistochemistry: Reported by independent researcher using representative lot.
Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
Immunohistochemistry: Reported by independent researcher using representative lot.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
Target description
~ 110 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lidong Sun et al.
Cell cycle (Georgetown, Tex.), 15(19), 2647-2655 (2016-10-18)
SIRT1 is a principle class III histone deacetylase which exhibits versatile functions in stress response, development, and pathological processes including cancer. Although SIRT1 deacetylates a wide range of nuclear and cytoplasmic proteins, its subcellular localization in cancer cells has been
Timothy M Robinette et al.
Nature communications, 11(1), 2814-2814 (2020-06-06)
Food consumption is fundamental for life, and eating disorders often result in devastating or life-threatening conditions. Anorexia nervosa (AN) is characterized by a persistent restriction of energy intake, leading to lowered body weight, constant fear of gaining weight, and psychological
Lili Qin et al.
Molecules (Basel, Switzerland), 25(22) (2020-11-19)
Resveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid
Zhenyu Li et al.
The Journal of biological chemistry, 284(16), 10361-10366 (2009-02-17)
SUV39H1 is a histone H3K9-specific methyltransferase important for heterochromatin formation, regulation of gene expression, and induction of senescence in premalignant cells. SUV39H1 forms a complex with SirT1, and its activity is stimulated by SirT1 binding. Here we present evidence that
Li Zhang et al.
Bioscience trends, 12(3), 282-290 (2018-06-29)
The migration and invasion of vascular smooth muscle cells (VSMCs) caused by advanced aging play an important role in diffuse intimal thickening, facilitate adverse arterial remodeling and contribute to the initiation and progression of cardiovascular diseases. The inhibitory function of
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