accuracy: 80-120% (minimum of 10 samples spiked with acceptable recovery)
technique(s)
ELISA: suitable single molecule counting: suitable
General description
Interleukin-21 (IL-21) is a cytokine with a potent regulatory effect on the immune system. Mainly produced by activated T-cells, IL-21 is responsible for the hematopoiesis of T-, B-, natural killer and dendritic cells. The production and differentiation of these effector cells may lead to an increased immunity against cancerous or virally infected cells. Thus, the role of IL-21 in the regulation of immuno-inflammatory responses has important implications for diseases such as multiple sclerosis, metastatic melanoma and metastatic renal cell carcinoma. Thus IL-21 will remain an attractive subject for drug development. Successful study outcomes will further establish IL-21 immunotherapy as an effective treatment for various cancers and inflammatory diseases.
The Single Molecule Counting (SMC®) Mouse IL-21 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Application
Research Category Inflammation & Immunology
Research Sub Category Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Mouse IL-21 Discovery Immunoassay unparalleled performance in the quantification of IL-21 in mouse serum.
Used to detect/quantify:IL-21
Components
Assay Buffer Mouse IL-21 Coating Antibody Assay Plate 10X PBS Blocking Buffer Standard Diluent Mouse IL-21 Detection Antibody Mouse IL-21 Standard SMC® Mouse IL-21 Immunoassay Kit Instructions 10X Wash Buffer Buffer C Elution Buffer B
Analysis Note
Low-end CV% Range: 0-20%|Low-end CV% Average: 10%
Legal Information
SMC is a registered trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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