03-0123-00

SMC^® Mouse IL-17A Discovery Immunoassay Kit

• Synonym(s): mouse IL 17A immunoassay kit, mouse IL-17A Single Molecule Counting Kit, mouse IL-17A ultra-sensitive immunoassay, mouse Interleukin 17A immunoassay kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• species reactivity: mouse
• Quality Level: 200
• manufacturer/tradename: SMC^®
• assay range: accuracy: 80-120%; (minimum of 10 samples spiked with acceptable recovery)
• technique(s): ELISA: suitable; single molecule counting: suitable

Description

General description

Interleukin-17A (IL-17A) is the founding member of a group of cytokines called the IL-17 family. There are several members of the IL-17 family, from IL-17A to IL-17F (IL-17E is also called IL-25). IL-17A is involved in mediating proinflammatory responses by inducing the production of many other cytokines, chemokines, and prostaglandins from many cell types. The increased expression of chemokines attracts other cells, including neutrophils. IL-17A has many physiological functions, such as airway remodeling. As a result of these roles, the IL-17 family has been linked to many immune/autoimmune related diseases, including rheumatoid arthritis (RA), asthma, allergic responses, lupus, allograft rejection and antitumor immunity.

The SMC^® Mouse IL-17A Discovery Immunoassay Kit contains all reagents required to perform the assays. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The Single Molecule Counting (SMC^®) technology gives the SMC^® Mouse IL-17A Discovery Immunoassay Kit unparalleled performance in the quantification of IL-17A in mouse serum.

Used to detect/quantify:IL-17A

Components

Assay Plate
10X PBS
Blocking Buffer
Standard Diluent
Mouse IL-17A Detection Antibody
Mouse IL-17A Standard
SMC^® Mouse IL-17A Immunoassay Kit Instructions
10X Wash Buffer
Buffer C
Elution Buffer B

Analysis Note

Low-end CV% Range: 0-20%|Low-end CV% Average: 11%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H317,H319,H360FD,H412
• pcodes: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects