03-0094-00

SMC^® Interleukin 7 (IL-7) Immunoassay Kit

• Synonym(s): IL 7 immunoassay kit, IL-7 Single Molecule Counting Kit, IL-7 ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• species reactivity: human
• manufacturer/tradename: SMC^®
• assay range: accuracy: 80-120%; (minimum of 10 samples spiked with acceptable recovery)
• technique(s): ELISA: suitable; single molecule counting: suitable

Description

General description

Interleukin-7 (IL-7) is an endogenous cytokine responsible for the proliferation of B- and T-lymphocytes. The active human form of IL-7 has 152 aa and is expressed by fibroblasts, stromal, dendritic and endothelial cells. IL-7 often acts in synergy with other cytokines. For example, IL-7 is found to have an additive effect on T-cell activation in combination with IL-12. However, IL-7 is not redundant, as abnormal function of IL-7 has been implicated in immune disorders such as common variable immunodeficiency, HIV and rheumatoid arthritis (RA). Thus, IL-7 is an excellent candidate for therapeutic development because of its crucial role in the modulation of innate immunity. Both activation and suppression of IL-7 may be of value in future therapeutic interventions.

The SMC^® IL-7 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-7 Immunoassay Kit unparalleled performance in the quantification of IL-7 in human EDTA plasma and serum.

Used to detect/quantify:IL-7

Components

IL-7 Coated Beads
Standard Diluent
IL-7 Detection Antibody
Assay Buffer
IL-7 Standard
10X Wash Buffer
Elution Buffer B
Buffer D
SMC^® IL-7 Immunoassay Kit Instructions

Analysis Note

Low-end CV% Range: 1 - 20%|Low-end CV% Average: 15%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H317,H319,H360FD,H412
• pcodes: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects