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699200P

Avanti

Lipid A - Purified

Avanti Research™ - A Croda Brand

Synonym(s):

Lipid A Detoxified (Salmonella minnesotaR595)

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About This Item

CAS Number:
497156-01-1
UNSPSC Code:
12352211

assay

>99% (HPLC)

form

powder

packaging

pkg of 1 × 1 mg (699200P-1mg)
pkg of 1 × 5 mg (699200P-5mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

shipped in

dry ice

storage temp.

−20°C

SMILES string

O[C@H]([C@@H](CO[C@@H]([C@@H]1NC(C[C@H](OC(CCCCCCCCCCC)=O)CCCCCCCCCCC)=O)O[C@H](CO)[C@@H](OP([O-])(O)=O)[C@@H]1OC(C[C@H](O)CCCCCCCCCCC)=O)O[C@H](O)[C@@H]2NC(C[C@H](O)CCCCCCCCCCC)=O)[C@@H]2OC(C[C@H](O)CCCCCCCCCCC)=O.[NH4+]

General description

Monophosphoryl lipid A (MPLA) is produced by hydrolysis of endotoxin lipopolysaccharide (LPS) isolated from Salmonella minnesota R595. MPLA is purified from the hydrolysate and contains a mixture of structural analogs containing differing numbers of fatty acids and positional isomers.
Purified Lipid A (MPLA) is soluble in DMSO at ~1mg/mL. Alternatively, it has been reported that MPLA can be suspended at ~1mg/mL in water or isotonic saline containing 0.2% triethylamine. After adding water/saline containing triethylamine to the MPLA powder, swirl the suspension in 65-70°C water bath for 10-20 seconds, then disperse by sonication using an ultrasonic bath for 30 seconds. The final solution will be slightly turbid.

Application

Lipid A - purified has been used as an adjuvant in the reconstitution lipid mixture.[1]
Lipid A- purified has been used for toll-like receptor (TLR) ligand treatment.[2]

Biochem/physiol Actions

Detoxified monophosphoryl lipid A (MPLA) acts as a toll-like receptor 4 (TLR4) agonist. Hydrolysis of the lipopolysaccharide (LPS) to yield MPLA, retains the action of stimulating the adaptive immune response while eliminating many of the pro-inflammatory side effects experienced with LPS.

Packaging

2 mL Amber Glass Crimp Cap Vial (699200P-1mg)
2 mL Amber Glass Crimp Cap Vial (699200P-5mg)

Other Notes

For R&D use only. Not for drug, household, or other uses.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class

11 - Combustible Solids

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Sigma-Aldrich

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