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Key Documents

L020200

Sigma-Aldrich

TCA Deblock

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.21

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

packaging

pkg of 4 × 2 L L020200-04

concentration

28-32 g/L (TCA Content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear Appearance of Solution
clear

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Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system, Respiratory system

wgk_germany

WGK 2


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