L020080

TCA Deblock

configured for MerMade, configured for PerkinElmer, configured for Polygen

• UNSPSC Code: 12352200

Properties

• type: for DNA synthesis; for DNA synthesis
• product line: Proligo Reagents
• storage condition: dry at room temperature
• concentration: 28-32 g/L (TCA content, titration)
• impurities: ≤200 ppm water content (Karl Fischer)
• color: clear; clear
• compatibility: configured for MerMade; configured for PerkinElmer; configured for Polygen

Description

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H315,H318,H335,H336,H351,H411
• pcodes: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system, Respiratory system
• wgk_germany: WGK 2