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F15706

Sigma-Aldrich

Formamide

97%

Synonym(s):

Formamide, Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein/REAXYS Number:
505995
MDL number:
UNSPSC Code:
12352100
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vapor density

1.55 (vs air)

Quality Level

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

assay

97%

autoignition temp.

932 °F

refractive index

n20/D 1.447 (lit.)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

functional group

amide

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Formamide may be used as a solvent and a reducing agent in the preparation of anisotropic silver nanoparticles from silver perchlorate.[1]

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Synthesis of silver nanoprisms in formamide.
Sarkar A, et al.
Journal of Colloid and Interface Science, 287(2), 496-500 (2005)
Microwave spectrum, structure, dipole moment, and quadrupole coupling constants of formamide.
Kurland RJ and Wilson Jr EB.
J. Chem. Phys. , 27(2), 585-590 (1957)
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 19(2), 130-130 (1938)
Formamide can dramatically improve the specificity of PCR.
G Sarkar et al.
Nucleic acids research, 18(24), 7465-7465 (1990-12-25)
Anna A Perevalova et al.
Applied and environmental microbiology, 74(24), 7620-7628 (2008-10-14)
Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among

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