Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(10), 965-970 (2008-08-02)
Rapid flow cytometric measurement of the frequency of aneuploid human sperms is in increasing demand but development of an exploitable method is hindered by difficulties of stoichiometric staining of sperm DNA. An aggressive decondensation protocol is needed after which cell
Nuclear matrices were isolated from maize leaves by the two conventional methods usually employed for the preparation of the corresponding structures of animal origin. It is demonstrated that functionally competent matrices, recognizing and specifically binding the MAR-containing DNA of the
Archives of andrology, 15(2-3), 109-116 (1985-01-01)
Various methods of solubilization of human sperm antigens were compared by testing the sperm extracts against rabbit antihuman spermatozoa immune serums with double immunodiffusion and rocket immunoelectrophoresis. Marked differences were found in the protein contents and the SDS-PAGE protein patterns
Archives of andrology, 29(2), 117-126 (1992-09-01)
Sperm samples were collected from male partners of fertile and infertile couples having unexplained and male factor infertility and analyzed for morphological abnormalities. After a staining procedure, sperm samples of these infertile men showed a variety of abnormalities. The sperm
Five glycoproteinmolecules with the molecular masses of 17 000; 38 000; 42 000; 50 000 and 67 000 were purified by high performance liquid chromatography following solubilization of isolated porcine zonae pellucidae by treatment with lithium-3,5-diiodosalicylate. The N-terminal amino acid
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