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113832

Sigma-Aldrich

3,5-Diaminobenzoic acid dihydrochloride

99%

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About This Item

Linear Formula:
(H2N)2C6H3CO2H·2HCl
CAS Number:
618-56-4
Molecular Weight:
225.07
Beilstein/REAXYS Number:
3703362
EC Number:
210-556-8
MDL number:
MFCD00012983
UNSPSC Code:
12352200
PubChem Substance ID:
24847151
NACRES:
NA.22

assay

99%

reaction suitability

reaction type: solution phase peptide synthesis

mp

>300 °C (lit.)

application(s)

peptide synthesis

SMILES string

Cl[H].Cl[H].Nc1cc(N)cc(c1)C(O)=O

InChI

1S/C7H8N2O2.2ClH/c8-5-1-4(7(10)11)2-6(9)3-5;;/h1-3H,8-9H2,(H,10,11);2*1H

InChI key

IGPLRBRBTUNCRT-UHFFFAOYSA-N

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Application

Uses include the fluorometric microdetermination of DNA.[1]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
19322CY
05128PV
S35171
34880

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Interference of Bis-Tris buffer with the diaminobenzoic acid fluorescence assay used to quantify DNA.
W E Schy et al.
Mutation research, 226(4), 263-266 (1989-08-01)
T R Bosworth et al.
Analytical biochemistry, 223(2), 266-273 (1994-12-01)
Glycosaminoglycan (GAG) hexosamines were measured after deacetylation (2 h acid hydrolysis), deaminative cleavage by nitrous acid, and coupling of the 2,5-anhydro sugars thus produced with 3,5-diaminobenzoic acid (DABA) to give a fluorescent product. Analyses were performed after the addition of
Superoxide dismutase modification and genotoxicity of transition-metal ion chelators.
T P Coogan et al.
Advances in experimental medicine and biology, 197, 281-290 (1986-01-01)
C F Kuo et al.
Analytical biochemistry, 170(1), 183-185 (1988-04-01)
A method for staining iron proteins on polyacrylamide gel electropherograms is described. It is based upon catalysis, by iron, of the oxidation of diaminobenzoic acid by hydrogen peroxide. Bands containing as little as 5 ng of protein-bound iron can be
A rapid fluorometric method for the estimation of DNA in cultured cells.
B G Erwin et al.
Analytical biochemistry, 110(2), 291-294 (1981-01-15)
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