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Supelco

SUPELCOSIL ABZ+Plus (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL ABZ+Plus HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm

Agency

suitable for USP L60

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

12.0% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface area

170 m2/g

surface coverage

surface coverage 3.4 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL ABZ+Plus columns offer both high deactivation and unique selectivity. Deactivated silica particles of very narrow particle size distribution ensure high efficiency with low back pressure. After bonding and endcapping reactions, the ABZ+Plus phase effectively shields unreacted silanol groups on the silica, preventing them from interacting with most analytes, and provides symmetric peaks regardless of an analyte′s functionality. The phase also allows you to use low ionic strength buffers without having to add an ion-suppressing modifier. ABZ+Plus enables you to use simple mobile phases when analyzing the most difficult compounds; acids, strongly basic compounds, and zwitterions.
suitable for L60 per USP

Features and Benefits

• High efficiency for polar, nonpolar, and charged analytes
• Symmetric peaks for the most reactive compounds
• Unique selectivity for polar and charged compounds

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Tânia M G Almeida et al.
Chemistry & biodiversity, 2(12), 1691-1700 (2006-12-29)
Absorption, distribution, metabolism, and excretion (ADME) properties are of invaluable importance to a bioactive compound. Permeation process is one of the most widely studied by many different techniques. Among them, reversed-phase liquid chromatography (RP-LC) is proving to be of great
Zhongping John Lin et al.
Journal of pharmaceutical and biomedical analysis, 37(4), 757-762 (2005-03-31)
A highly sensitive method was developed and validated for determining the free fraction of flunarizine in human plasma. Equilibrium dialysis was used for the separation of free (unbound) drug and liquid chromatography/tandem mass spectrometry (LC-MS/MS) was used for quantitation. Post-dialysis
P Chaimbault et al.
Journal of chromatography. A, 855(1), 191-202 (1999-10-09)
A qualitative determination of 20 underivatized proteinic amino acids by LC-MS is reported. The need for chromatographic separation before mass spectrometry determination is demonstrated based on the study of several amino acid pairs which have some similar characteristics. Two suitable
W Pan et al.
Journal of chromatography. B, Biomedical sciences and applications, 703(1-2), 129-138 (1998-02-03)
A sensitive, specific and precise HPLC-UV assay was developed to quantitate cocaine (COC) and its metabolites benzoylecgonine (BE), norcocaine (NC) and cocaethylene (CE) in rat plasma. After adding 50 microl of the internal standard solution (bupivacaine, 8 microg/ml) and 500
K Ary et al.
Journal of chromatography. A, 797(1-2), 221-226 (1998-05-16)
A reversed-phase high-performance liquid chromatographic method with electrochemical detection has been developed for the determination of buspirone from human plasma. The separation was carried out by using a Supelcosil ABZ+ plus C18 reversed-phase column and 0.05 M potassium dihydrogenphosphate (pH

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