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T5569

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB substrate for ELISA (Super Slow)

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate

form

liquid

shipped in

wet ice

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Roberto Lande et al.
Antibodies (Basel, Switzerland), 9(2) (2020-05-15)
Human cathelicidin LL37 is a cationic antimicrobial peptide active against bacteria and viruses and exerting immune modulatory functions. LL37 can be also a target of autoreactive B- and T-lymphocytes in autoimmune settings. Irreversible post-translational modifications, such as citrullination and carbamylation
Thomas J Esparza et al.
bioRxiv : the preprint server for biology (2021-11-19)
There remains an unmet need for globally deployable, low-cost therapeutics for the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Previously, we reported on the isolation and in vitro characterization of a potent single-domain nanobody, NIH-CoVnb-112, specific for the
Dimitris Missirlis et al.
Biophysical journal, 119(12), 2558-2572 (2020-11-21)
The mechanics of fibronectin-rich extracellular matrix regulate cell physiology in a number of diseases, prompting efforts to elucidate cell mechanosensing mechanisms at the molecular and cellular scale. Here, the use of fibronectin-functionalized silicone elastomers that exhibit considerable frequency dependence in
Anil G Cashikar et al.
Journal of lipid research, 64(4), 100350-100350 (2023-02-28)
Neuroinflammation, a major hallmark of Alzheimer's disease and several other neurological and psychiatric disorders, is often associated with dysregulated cholesterol metabolism. Relative to homeostatic microglia, activated microglia express higher levels of Ch25h, an enzyme that hydroxylates cholesterol to produce 25-hydroxycholesterol
ApoE4 accelerates early seeding of amyloid pathology
Liu CC, et al.
Neuron, 96(5), 1024-1032 (2017)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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