SRP3084

IL-1Ra human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): ICIL-1RA, IL-1RN, IRAP
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 20-40 ng/mL
• mol wt: 17.2 kDa
• packaging: pkg of 100 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• suitability: suitable for molecular biology
• UniProt accession no.: P18510
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL1R1(3554)

Description

General description

Research Area: IMMUNO AND CKS
The interleukin-1 receptor antagonist (IL-1Ra) is part of the IL-1 family and binds to IL-1 receptors without triggering any intracellular response. IL-1Ra, produced by hepatic cells, exhibits characteristics of an acute phase protein. Endogenous IL-1Ra is generated in various experimental animal models of disease, as well as in human autoimmune and chronic inflammatory conditions.

The gene IL-1RA (interleukin-1 receptor antagonist) is mapped to human chromosome 2q. It belongs to the IL-1 (interleukin 1) family of proteins. The encoded protein has a TIR (Toll/IL-1 receptor) domain. IL-1RA is produced by corneal epithelial cells, monocytes, neutrophils, macrophages and fibroblasts. It is a naturally occurring inflammatory inhibitor protein. Recombinant human IL-1RA is a 17.2kDa protein consisting of 153 amino acid residues.

Application

IL-1Ra human has been used as an IL1 antagonist to block the signaling from the IL1 receptor and verify that IL1 signaling is responsible for invasiveness in CD133-expressing cells. It has also been used as a control in sandwich enzyme-linked immunosorbent assay (ELISA) to measure protein levels.

Biochem/physiol Actions

Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α (interleukin 1 α) and IL-1β (interleukin 1 β) by competitively blocking binding to their type I and type II receptors. Therapeutically, IL-1RA may help in the treatment of sepsis, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Low production of IL-1RA is observed in HIV-1 (human immunodeficiency virus 1)-infected dendritic cells.

The interleukin-1 receptor antagonist (IL-1Ra) functions as a specific and selective competitive inhibitor of IL-1R1, binding to the receptor with high affinity.

Sequence

MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE

Physical form

Lyophilized from 5mM Tris, pH 8.0 + 25 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable