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SAB4200466

Sigma-Aldrich

Anti-Desmoglein 2 (DSG2) antibody, Mouse monoclonal

clone AH12.2, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-ARVC10, Monoclonal Anti-ARVD10, Monoclonal Anti-CDHF5, Monoclonal Anti-CMD1BB, Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse, Monoclonal Anti-HDGC, Monoclonal Anti-desmoglein 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AH12.2, monoclonal

mol wt

antigen ~150 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa total cell extracts

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DSG2(1829)

General description

Monoclonal Anti-Desmoglein 2 (DSG2) (mouse IgG1 isotype) is derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. Desmogleins are a family of cadherin proteins. There are four distinct desmoglein genes (DSG1-DSG4), each differentially expressed depending upon the type of tissue and the state of differentiation. Desmoglein-2 (DSG2) is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis and heart.

Immunogen

derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells.

Application

Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Desmogleins play an important role in the formation of desmosomes, cell to-cell adhesions, often found in both simple and squamous epithelium. These adhesions are important for ensuring tissue integrity and regulation of paracellular movement of solutes and restricting access of luminal pathogens to underlying tissue compartments. Desmoglein-2 (DSG2) has been shown to regulate numerous cellular processes, including proliferation and apoptosis. Intracellular fragments of DSG2 promote apoptosis in colonic epithelial cells. Mutations in this gene is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutations in desmoglein-2 gene are associated with arrhythmogenic right ventricular cardiomyopathy
Pilichou K, et al.
Circulation, 113(9), 1171-1179 (2006)
Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines
Kolegraff K, et al.
Cell adhesion & migration, 5(4), 306-314 (2011)
Desmoglein as a target in skin disease and beyond
Amagai M, and John RS
The Journal of Investigative Dermatology, 132(3), 776-784 (2012)
Lisa Leon Gallegos et al.
Scientific reports, 6, 27114-27114 (2016-06-04)
Cell-cell adhesion is central to morphogenesis and maintenance of epithelial cell state. We previously identified 27 candidate cell-cell adhesion regulatory proteins (CCARPs) whose down-regulation disrupts epithelial cell-cell adhesion during collective migration. Using a protein interaction mapping strategy, we found that

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