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Key Documents

RAB0510

Sigma-Aldrich

Mouse VEGF ELISA Kit

for cell and tissue lysates

Synonym(s):

VEGF-A

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Vegfa(22339)

General description

The Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF cell lysate and tissue lysate.

Immunogen

Recombinant Mouse VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse VEGF ELISA Kit has been used to determine the concentration of VEGF expression in samples using ELISA.

Biochem/physiol Actions

Vascular endothelial growth factor (VEGF) is a critical factor in regulating angiogenesis in both physiological and pathological conditions. Transforming growth factor-α (TGF-α), TGF-β and platelet derived growth factor can potentially activate VEGF activity. VEGF mediates its activity through tyrosine kinase receptors, vascular endothelial growth factor receptor-1 (VEGFR-1) and VEGFR-2. VEGF produced by tumour cells and macrophages, plays a key role in cancer development by promoting endothelial cells proliferation and increasing vascular permeability. Enhanced expression of VEGF plays a key role in urologic neoplasm like bladder cancer, renal cell carcinoma, and cervical cancer. Polymorphism in VEGF gene increases the disease susceptibility. Blocking of VEGF mediated signalling pathway is a potential therapeutic strategy for cancer patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sabbah DA, et al.
Medicinal Chemistry, 14, 1-14 (2018)
The crucial role of blood VEGF kinetics in patients with locally advanced esophageal squamous cell carcinoma receiving curative concurrent chemoradiotherapy
Chen YH, et al.
BMC Cancer, 18(1), 837-837 (2018)
Susinder Sundaram et al.
Journal of molecular medicine (Berlin, Germany), 96(11), 1177-1187 (2018-08-30)
Endothelial cells respond to mechanical stimuli such as stretch. This property can be exploited with caution to induce angiogenesis which will have immense potential to treat pathological conditions associated with insufficient angiogenesis. The primary aim of this study is to
Stimulation of angiogenesis using single-pulse low-pressure shock wave treatment
Sundaram S, et al.
Journal of Molecular Medicine, 96(11), 1177-1187 (2018)
Fu-Li Xiang et al.
Circulation. Heart failure, 7(5), 831-842 (2014-08-12)
The adult epicardium is a potential source of cardiac progenitors after myocardial infarction (MI). We tested the hypothesis that cardiomyocyte-specific overexpression of membrane-associated human stem cell factor (hSCF) enhances epicardial activation, epicardium-derived cells (EPDCs) production, and myocardial arteriogenesis post MI.

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