Skip to Content
Merck
All Photos(1)

Documents

N3755

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym(s):

Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9013-53-0
Enzyme Commission number:
3.1.31.1 (BRENDA, IUBMB)
EC Number:
232-748-0
MDL number:
MFCD00131731
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Staphylococcus aureus

Quality Level

300

form

lyophilized powder

specific activity

100-300 units/mg protein

composition

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

technique(s)

DNA purification: suitable

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Joana Segura et al.
Nature communications, 9(1), 3989-3989 (2018-09-30)
The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service