Skip to Content
Merck
All Photos(1)

Key Documents

MSST0002

Sigma-Aldrich

SILuLite APOA1 Apolipoprotein A-1 human

recombinant, expressed in HEK 293 cells, MS Protein Standard

Synonym(s):

SILuLite Apolipoprotein A-1

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23201100
NACRES:
NA.12

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

tag

His tagged
V5 tagged

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

packaging

vial of 50-65 μg (Lot-specific vial content given on certificate of analysis)

technique(s)

mass spectrometry (MS): suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... APOA1(335)

Related Categories

Biochem/physiol Actions

Apolipoprotein A-1(Apo-A1), is a major protein component of high density lipoprotein (HDL)in plasma1. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters2. Apo-A1 was shown to assist in the improvement of predicting cardiovascular events3. The gene that encodes to Apolipoprotein A-1 is APOA1. This gene is closely linked with two other apolipoprotein genes on chromosome 114. Defects in this gene are associated with HDL deficiencies, including Tangier disease5, and with systemic non-neuropathic amyloidosis6. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-A1 shows a high content of a-helix structure6. The amphipathic regions in the α-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-A1 shows self-association with minor conformational change7. In addition, it has been shown that Apo-A1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin8.

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Analysis Note

General Description

SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.

Sequence

RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ

The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized.

Other Characterization

  • Sequence confirmed by intact mass analysis
  • Identity verified by peptide mapping
  • purity >98% (SDS-PAGE)
  • Vial content was determined by the Bradford method using BSA as a calibrator. The correction factor of Bradford-to-AAA is 88.33%


Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

B Lamarche et al.
Clinica chimica acta; international journal of clinical chemistry, 286(1-2), 145-161 (1999-10-08)
Reduced plasma high-density lipoprotein (HDL) cholesterol levels have been recognized as a highly significant independent risk factor for atherosclerotic cardiovascular disease. HDL levels are also inversely related to plasma triglyceride levels and there is a dynamic interaction between HDL and
E M Rubin et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(2), 434-438 (1991-01-15)
In Western societies high density lipoprotein (HDL) levels correlate inversely with the risk for coronary heart disease. The primary protein component of both human and mouse HDL is apolipoprotein A-I (apoAI), which comprises greater than 70% of HDL protein and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service