MITOISO1

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

• Synonym(s): Mitochondria Purification Kit
• EC Number: 232-650-8
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• Quality Level: 200
• usage: sufficient for 10-20 g (animal tissue); sufficient for 50 assays (2 mL)
• shelf life: 2 yr
• technique(s): fractionation: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Mitochondria Isolation Kit has been used:

• for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
• for the isolation of mitochondria from glomeruli and heart
• for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

• Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
• Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
• Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
• Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Kit Components Also Available Separately

• T9201 Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids