This kit is not suitable for use in non-cellular environments and is intended only for the analysis of live cells as described in the Technical Bulletin.
MAK144
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (orange)
Synonym(s):
Fluorometric ROS Assay Kit
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About This Item
Pricing and availability is not currently available.
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usage
sufficient for 200 fluorometric tests (orange)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
cancer; neurological disorders
storage temp.
−20°C
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell.[1] At physiological levels, ROS contribute to cell signaling and host defense.[2] Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1][2] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[3]
Application
Suitability
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.
Principle
The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 540/λem = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Does it work for non-cellular environments, like a solution? Thanks.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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