L8777 is recommended for cell culture because it is a sterile product.
L9379
Lectin from Phytolacca americana (pokeweed)
lyophilized powder
Synonym(s):
PWM, Pokeweed mitogen
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
Recommended Products
sterility
aseptically filled
Quality Level
form
lyophilized powder
potency
≤0.3 μg/mL mitogenic activity
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.[1]
Application
Lectin from Phytolacca americana (pokeweed) has been used:
- as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation[2][3]
- as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes[4]
- as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures[5]
Biochem/physiol Actions
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Physical form
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Analysis Note
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism
Demyda P, et al.
Theriogenology, 81(8), 1116-1122 (2014)
B Hejdeman et al.
HIV medicine, 4(2), 101-110 (2003-04-19)
To study the impact of effective highly active antiretroviral therapy (HAART) on the preservation of long-term CD4 memory cells induced by vaccines in HIV-1-infected patients. Thirty HIV-1-positive patients on HAART with undetectable viral load were randomized into three groups: 10
Assessment of cellular immune responses of healthy and diseased Tasmanian devils (Sarcophilus harrisii)
Kreiss A, et al.
Developmental and Comparative Immunology, 32(5), 544-553 (2008)
W E Müller et al.
European journal of biochemistry, 183(2), 391-396 (1989-08-01)
Previously it was established [Pahwa, S., Pahwa, R., Saxinger, C., Gallo, R. C. & Good, R. A. (1985) Proc. Natl. Acad. Sci. USA 82, 8198] that nonviable preparations of human immunodeficiency virus 1 (HIV-1) abolish the proliferative response of human
The immune response of the Tasmanian devil (Sarcophilus harrisii) and devil facial tumour disease
Woods GM, et al.
EcoHealth, 4(3), 338-345 (2007)
Articles
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
-
Is the product Pokeweed L9379 suitable for cell cultures from B lymphocytes? Or is better to use the product L8777? We culture bone marrow samples from B-cell dyscrasias for cytogenetic studies.
1 answer-
Helpful?
-
-
how to prepare Pokeweed to be used as mitogen for B-ALL cytogenetics blood culture
1 answer-
This product can be dissolved in PBS and used at a concentration of up to 3 µg/mL for a mitogenic effect on human peripheral blood mononuclear cells. Concentrations higher than 3 µg/mL may be toxic to these cells.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
