When the Gill No 1 hematoxylin is first poured from the bottle, the solution appears as a dark reddish-purple color. If the nuclei appear purple instead of blue after staining, it suggests that the slides were not adequately blued. The Instructions for Use advise using Scott's Tap Water Substitute (catalog number S5134). If warm running tap water was used, it's likely that the slides were not washed for a sufficient duration to convert the purple color to blue. If Scott's Tap Water Substitute was used, it's possible that the old solution needs to be replaced with a freshly diluted 1X solution from the 10X concentrate. Although the official recommendation is for Scott's Tap Water Solution, other blueing solutions are commonly used with different hematoxylin solutions. For instance, 15 grams of sodium bicarbonate per liter of tap or deionized water can be used, or a saturated solution of lithium carbonate. Some have also used a few drops of concentrated ammonium hydroxide in 500 ml of tap or deionized water in the past, but it's important to avoid using too much as it can strip the tissue sections from the glass slides. Typically, the slides are left in the blueing solution until the tissue sections appear blue and not purple. Once the tissue has turned blue, it should be thoroughly washed in running tap water to remove any traces of the blueing solution. Proper removal of the blueing solution is crucial, as leaving traces on the slides will lead to poor staining with Eosin Y solution.
Recommended Products
form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
2 g/L
technique(s)
microbe id | staining: suitable
pH
2.5-3.5
application(s)
hematology
histology
storage temp.
room temp
SMILES string
Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
InChI
1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
InChI key
WZUVPPKBWHMQCE-XJKSGUPXSA-N
Looking for similar products? Visit Product Comparison Guide
Application
Other Notes
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - STOT RE 2 Oral
Target Organs
Kidney
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Related Content
Brief information on the field of cytology, its role in cytodiagnosis and the use of standard and special stains for microscopic analysis to detect cellular abnormalities.
Brief information on the field of cytology, its role in cytodiagnosis and the use of standard and special stains for microscopic analysis to detect cellular abnormalities.
Brief information on the field of cytology, its role in cytodiagnosis and the use of standard and special stains for microscopic analysis to detect cellular abnormalities.
Brief information on the field of cytology, its role in cytodiagnosis and the use of standard and special stains for microscopic analysis to detect cellular abnormalities.
-
When using SLCH6216, there is light purple/violet staining of nuclei with a gray background in the cytoplasm. Increasing the staining time up to 15 minutes does not result in increased stain intensity. How can this issue be addressed?
1 answer-
Helpful?
-
-
혹시 해당 제품의 대체품을 추천해 주실 수 있을까요?
1 answer-
The direct replacements for GHS132(1L) would be GHS316 (500mL) and GHS1128(4L). There are other similar formulations that may be of interest. See the list below. Each option includes a link to the product datasheet and the product list for that formulation, respectively.
1. Gill No. 2
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/620/308/procedure-ghs-hematoxylin-stains-ms.pdf
https://www.sigmaaldrich.com/US/en/substance/hematoxylinsolutiongillno21234598765
2. Gill No. 3
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/620/308/procedure-ghs-hematoxylin-stains-ms.pdf
https://www.sigmaaldrich.com/US/en/substance/hematoxylinsolutiongillno31234598765
3. Mayer's Hematoxylin
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/066/procedure-mhs-mayers-hematoxylin-solution-ms.pdf
https://www.sigmaaldrich.com/US/en/substance/mayershematoxylinsolution1234598765
4. Harris Hematoxylin
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/239/767/procedure-hhs-harrishematoxylinsolution-ms.pdf
https://www.sigmaaldrich.com/US/en/substance/harrishematoxylinsolutionmodified1234598765Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
