FBS25

Fast Blue RR Salt

Preweighed Capsules

• Synonym(s): 4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24
• Linear Formula: C₁₅H₁₄ClN₃O₃ · 1/2 ZnCl₂
• CAS Number: 14726-29-5
• Molecular Weight: 387.89
• Colour Index Number: 37155
• EC Number: 238-777-5
• MDL number: MFCD00074762
• UNSPSC Code: 12171500
• PubChem Substance ID: 24895004
• NACRES: NA.47

Properties

• form: powder
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• application(s): hematology; histology
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
• InChI: 1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
• InChI key: LLARVKAOQLUCQI-UHFFFAOYSA-L

Description

General description

Fast Blue RR is part of the azo coupling system and is required for the staining of hydrolytic enzymes. In the presence of naphthol, it results in the formation of an insoluble coloured precipitate at the site of enzyme activity.

Application

Used as a kit component for the determination of alkaline phosphatase in Sigma Kits 85L1 and 85L2 and for the determination of nonspecific esterase in Sigma Kit 90A1.
Fast Blue RR Salt has been used for the staining of alkaline phosphatase activity in cells.

Packaging

Actual weight of the capsule will vary with dye lot and has been optimized by assay.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves