Skip to Content
Merck
All Photos(1)

Key Documents

MEWO

93082609, human skin, Fibroblast

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

product name

MEWO, 93082609

biological source

human skin

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Melanin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human malignant melanoma

Cell Line Description

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

Application

Virus studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service