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C6409

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD00162222
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

chicken

technique(s)

indirect ELISA: 1:150,000-1:250,000
quantitative precipitin assay: 2.5-5 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Chicken IgG (immunoglobulin G) is also referred to as IgY. IgY shows similarity to mammalian IgG, differing by the presence of one more constant region domain and absence of functional hinge regions. IgY is the most abundant serum immunoglobulin in birds.

Application

Anti-Chicken IgY (IgG) (whole molecule) antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

IgY antibody subtype is the most abundant of serum immunoglobulins of the immune system in birds and is analogous to IgG found in mammals. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Site-specific N-glycosylation of chicken serum IgG
Suzuki N and Lee YC
Glycobiology, 14(3), 275-292 (2003)
It?s gut check time! A universal food immunomarking technique for studying arthropod feeding activities
Hagler JR, et al.
Annals of the Entomological Society of America, 112(3), 211-219 (2019)
Suvi Ruuskanen et al.
PloS one, 6(11), e25360-e25360 (2011-11-24)
Reproductive, phenotypic and life-history traits in many animal and plant taxa show geographic variation, indicating spatial variation in selection regimes. Maternal deposition to avian eggs, such as hormones, antibodies and antioxidants, critically affect development of the offspring, with long-lasting effects
A Larsson et al.
Poultry science, 72(10), 1807-1812 (1993-10-01)
Laying hens are highly cost-effective as producers of antibodies compared with the mammals traditionally used for such production. Also, chicken antibodies have biochemical advantages over mammalian antibodies due to the phylogenetical differences between avian and mammalian species, resulting in increased
Immunoglobulins of the non-galliform birds: antibody expression and repertoire in the duck.
Lundqvist ML
Developmental and Comparative Immunology, 30(1-2), 93-100 (2006)
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