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C2821

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–Cy3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

goat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

The product binds to all goat Igs.

Application

Anti-Goat IgG (whole molecule)−Cy3 antibody produced in rabbit has been used in immunofluorescent labeling.
Anti-Goat IgG (whole molecule)-Cy3 antibody produced in rabbit has been used for immunofluorescent staining and immunohistochemistry of formalin-fixed, paraffin-embedded sections at a working dilution of 1:300. It has also been used in immunocytochemistry of mouse pancreatic islet cells.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Goat IgG (whole molecule) antibody allows for the visualization of proteins by fluorescent microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Renata L Fleming et al.
Journal of neurochemistry, 124(5), 621-631 (2012-12-12)
Pituitary Adenylyl Cyclase-Activating Polypeptide (PACAP) is a neuroactive peptide present in the avian retina where it activates adenylyl cyclase (AC) since early in development via PACAP receptors. The synthesis of cAMP in response to PACAP is observed since embryonic day
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Zhou B, et al.
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The apoA-I/C-III/A-IV gene cluster, like most intestine-specific genes, displays a specific pattern of expression along the intestinal cephalocaudal and crypt-to-villus axes. We have shown that this specific pattern of expression requires the distal apoA-IV promoter and the apoC-III enhancer. Using
Gina M Northington et al.
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Krawiec JT, et al.
Journal of Vascular Surgery, 66(3), 883-890 (2017)

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