A4418

Ammonium sulfate

for molecular biology, ≥99.0%

• Synonym(s): Mascagnite
• Linear Formula: (NH₄)₂SO₄
• CAS Number: 7783-20-2
• Molecular Weight: 132.14
• EC Number: 231-984-1
• MDL number: MFCD00003391
• UNSPSC Code: 12352314
• eCl@ss: 38030408
• PubChem Substance ID: 24890861
• NACRES: NA.31
• Assay: ≥99.0%
• grade: for molecular biology
• form: powder
• solubility: H₂O: 0.667 g/mL

Properties

• grade: for molecular biology
• Quality Level: 200
• Assay: ≥99.0%
• form: powder
• technique(s): affinity binding assay: suitable; protein purification: suitable
• impurities: ≤0.005% Insolubles
• pH: 5.0-6 (25 °C, 132 g/L)
• mp: >280 °C (dec.) (lit.)
• solubility: H₂O: 0.667 g/mL
• density: 1.77 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 ppm
• cation traces: Cu: ≤5 ppm; Fe: ≤5 ppm; Zn: ≤5 ppm; heavy metals (as Pb): ≤2 ppm
• absorption: ≤0.025 at 280 at 1 M; ≤0.03 at 260 at 1 M
• suitability: suitable for flow cytometry; suitable for molecular biology
• application(s): metabolomics; microbiology
• foreign activity: DNase, RNase, and protease, none detected
• SMILES string: N.N.OS(O)(=O)=O
• InChI: 1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
• InChI key: BFNBIHQBYMNNAN-UHFFFAOYSA-N

Description

General description

Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins.[1] Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media.[2] Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.

Application

Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used

• in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins. [3]
• to precipitate the Shiga-like toxin from the culture supernatant.[4]
• for precipitaion of viral particles from the culture supernatant. [5]

Features and Benefits

• Heavy metals (as Pb): ≤2 ppm
• Free from DNase, RNase, and protease

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves