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A2792

Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 29:1 (ratio)

Synonym(s):

Acrylamide/Bisacrylamide, 29:1 (3.3% crosslinker), Polyacrylamide solution

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About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770795
NACRES:
NA.25
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

feed ratio

29:1 (ratio)

foreign activity

DNase, RNase and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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General description

Acrylamide (AA) is a neurotoxin and carcinogen. It was found in potato products like, french fries and potato chips and also in several cereal-based products.[1] This highly reactive organic compound has the ability to polymerize to form polyacrylamide.[2]

Application

Acrylamide/Bis-acrylamide has been used in the preparation of polyacrylamide (PA) gels.[3] It has also been used as a component in the preparation of blotting gel precursor solution, separation gel precursor solution and loading gel precursor solution.[4]
Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000374793
0000301088
0000301088
0000374794
0000374793

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Automated microfluidic protein immunoblotting
He M and Herr AE
Nature Protocols, 5(11), 1844-1844 (2010)
Use of potato flour in bread and flat bread
Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)
Acrylamide: a common food toxin related to physiological functions and health
Semla M, et al.
Physiological Research, 66(2), 205-205 (2017)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions
Chiang MYM, et al.
Biomaterials, 34(38), 9754-9762 (2013)
Yadong Wang et al.
Ecotoxicology and environmental safety, 175, 110-117 (2019-03-22)
Cadmium (Cd) is a known human carcinogen. Previous studies have demonstrated that Cd exposure promoted migration and invasion of breast cancer cells. However, the molecular mechanisms underlying this process have not yet been clearly addressed. The purpose of this study
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Questions

  1. I purchased the item A2792-100ML, however, there are no instructions on how to reconstitute this so that I have a 30% acrylamide/bis solution. Where can I find instructions?

    1 answer

    1. The instructions on how to use Product A2792 to make 100 mL of a 40 percent solution are provided on the Product Detail Page in the DESCRIPTION section under the heading of Reconstitution:

      "To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution."

      To use this 100 mL of the 40% solution to in turn make a 30% solution, add additional distilled or deionized water to bring the total volume to 133 mL; the total volume can be measured in a graduated cylinder. It should take roughly 30 mL of additional water, but the total volume (133 mL) needs to be measured accurately.
      .
      Alternatively, it be more convenient to order the Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1, which we list as Product A3574. Please see the link below to review this product:
      https://www.sigmaaldrich.com/product/sigma/a3574.

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