90107

Gram′s iodine solution

for microscopy

• Synonym(s): Potassium Triiodide
• UNSPSC Code: 12171500
• NACRES: MA.02

Properties

• grade: for microscopy
• Quality Level: 100
• product line: BioChemika
• form: liquid
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• color: brown to very dark brown
• refractive index: n20/D 1.335
• density: 1.005 g/mL at 20 °C
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• suitability: bacteria

Description

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves