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415049

Sigma-Aldrich

Aniline Blue diammonium salt

certified by the Biological Stain Commission

Synonym(s):

Ink Blue R; 4-[4-[[4-(4-aminooxysulfonylanilino)phenyl]-[4-(4-aminooxysulfonylphenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C37H32N5O9S3
CAS Number:
66687-07-8
Molecular Weight:
786.87
UNSPSC Code:
12171500
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder, crystals or granules

composition

Dye content, 65%

color

brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown

ε (extinction coefficient)

15000 at 595-605 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C37H31N5O9S3/c38-50-53(46,47)35-21-15-32(16-22-35)41-29-9-3-26(4-10-29)37(25-1-7-28(8-2-25)40-31-13-19-34(20-14-31)52(43,44)45)27-5-11-30(12-6-27)42-33-17-23-36(24-18-33)54(48,49)51-39/h1-24,40-41H,38-39H2,(H,43,44,45)

InChI key

CSYADFXDZYPANK-UHFFFAOYSA-N

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General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. It is a mixture of methyl blue (Cl 42780, Acid blue 93) and water blue (Cl 42755, Acid blue 22).

Application

Aniline blue (nominally as methyl blue or water blue) is a component of commonly used polychrome stains. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Suitability

Certified for use in Mallory′s connective tissue stain and Gomori′s one-step trichrome.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBL0699
MKCF9744
MKBZ0017V
MKBV0650V
MKBR5157V

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Herburger K and Holzinger A
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Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration.
Marro J, et al.
PLoS ONE, 11(10) (2016)
Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming
Sanchez-Ortiz A C, et al.
Latin American Journal of Aquatic Research, 43(1), 123-136 (2015)
Jan Marro et al.
PloS one, 11(10), e0165497-e0165497 (2016-10-27)
Zebrafish heart regeneration depends on cardiac cell proliferation, epicardium activation and transient reparative tissue deposition. The contribution and the regulation of specific collagen types during the regenerative process, however, remain poorly characterized. Here, we identified that the non-fibrillar type XII
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