1.52271
Chromolith® WP 300 RP-18 (monolithic) HPLC Columns
L × I.D. 50 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 RP-18 50-4.6 HPLC column
About This Item
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product name
Chromolith® WP 300 RP-18, L × I.D. 50 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped: no
endcapped: no
packaging
1 ea of
extent of labeling
18% Carbon loading
18% loading
parameter
200 bar pressure
200 bar pressure
60 °C max. temp.
60 °C max. temp.
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable
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General description
Accurate analysis of proteins, antibodies, and large peptides requires columns with good permeability, along with better mass transfer and selectivity. In order for size-exclusion not to influence the separation, the pore size should be approximately ten times larger than the molecule being analyzed.Chromolith columns have shown great potential and superiority in comparison to standard silica. Chromolith® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® WP 300 RP-18 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), and the surface modification is Octadecylsilane. Chromolith® WP 300 RP-18 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® WP 300 RP-18 columns are suitable for LC-MS applications.The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for very fast separations of Biomolecules on any HPLC System.Chromolith® WP 300 RP-18 50-4.6 HPLC columns enable:
- Completely bioinert column hardware and high biorecovery
- High-speed separations at very low column back-pressure
- High matrix tolerance, allowing time and cost savings with simplified workflows
- Cost-savings due to extended column lifetimes
- Connection of columns, flow gradients, and the use of mobile phases with higher viscosity
Legal Information
Certificates of Analysis (COA)
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