14-439-M

MAP Kinase 1/Erk1 Protein, active, 10 µg

Active, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays.

• Synonym(s): ERT1, Extracellular signal-regulated kinase 2 (ERK-2), MAPK 1, Mitogen-activated protein kinase 1, p44 MAPK
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• form: liquid
• mol wt: M_w 69.9 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• color: colorless
• solubility: water: soluble
• NCBI accession no.: NM_002745.4; NM_138957.3
• UniProt accession no.: P28482
• Gene Information: human ... MAPK3(5595)

Description

General description

Research area: Cell Signaling

Recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag

Biochem/physiol Actions

Extracellular-signal-regulated kinase 1 (ERK1)/ Extracellular-signal-regulated kinase 2 (ERK2), as part of the Ras-Raf-MEK-ERK signal transduction cascade, are involved in regulating a wide range of cellular processes, including cell adhesion, cell cycle progression, cell migration, cell survival, differentiation, metabolism, proliferation, and transcriptional control. Additionally, ERK1 signaling contributes to the regulation of cyclin D1 expression during the cell cycle. MAP kinases play a crucial role in inflammation and immune responses by regulating the production of pro- and anti-inflammatory cytokines, as well as prostaglandins, in response to lipopolysaccharide (LPS) stimulation.

Packaging

Also available in 250μg size as catalog # 14-439M - Call for pricing and availability.

Quality

routinely evaluated by phosphorylation of MBP

Physical form

Purified by glutathione-agarose. Activated with MEK1 and repurified on Ni2+/NTA agarose

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2