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14-420

Sigma-Aldrich

MEK1 Protein, inactive, 50 g

Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

Synonym(s):

MEK1 Protein

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

recombinant

expressed in E. coli

mol wt

Mw 71 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... MAP2K1(5604)

General description

Research area: CELL SIGNALING

N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1

Product Source: expressed in E.coli

MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.

Application

Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:
  • to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways.
  • to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).
  • to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs).

Biochem/physiol Actions

Protein Target: MEK1

Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.

Quality

routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro

Physical form

glutathione-agarose followed by Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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MEK1/2 dual-specificity protein kinases: Structure and regulation
Roskoski R Jr.
Biochemical and Biophysical Research Communications, 417(1), 5-10 (2012)
Jeeyun Lee et al.
Journal of the National Cancer Institute, 103(8), 674-688 (2011-03-15)
Metastatic colorectal cancer (CRC) patients with v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are resistant to treatment with cetuximab, a monoclonal antibody that targets the epidermal growth factor receptor. Statins have reported antitumor activity, but it is unknown
Langerhans Cell Histiocytosis
Diagnostic Pathology (2016)
MEKK2 mediates aberrant ERK activation in neurofibromatosis type I
Bok S, et al.
Nature Communications, 11(1), 5704-5704 (2020)
Non-V600 BRAF mutations recurrently found in lung cancer predict sensitivity to the combination of Trametinib and Dabrafenib
Noeparast A, et al.
Oncotarget, 8(36), 60094-60108 (2016)

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