M041130
6-Fluorescein Phosphoramidite
configured for ABI
Synonym(s):
6-FAM-phosphoramidite
About This Item
type
for DNA synthesis
Quality Level
product line
Proligo Reagents
Assay
≥97% (31P-NMR)
≥97.0% (reversed phase HPLC)
color
solid white to off-white
solubility
acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)
λ
conforms (UV/VIS Identity)
suitability
conforms to structure for H-NMR
compatibility
configured for ABI
storage temp.
−20°C
SMILES string
CC(C)N(C(C)C)P(OCCCCCCNC(=O)c1ccc2C(=O)OC3(c4ccc(OC(=O)C(C)(C)C)cc4Oc5cc(OC(=O)C(C)(C)C)ccc35)c2c1)OCCC#N
InChI
1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-34-37(26-31)46(59-41(34)51)35-20-17-32(56-42(52)44(5,6)7)27-38(35)58-39-28-33(18-21-36(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
InChI key
MGPYJVWEJNTXLC-UHFFFAOYSA-N
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General description
- 6-Fluorescein Phosphoramidite consists of one isomer derived from6-carboxy fluorescein
- Couples to the 5′-end of the oligonucleotide using standard synthesisprotocols
- Protecting groups on the fluorescein moiety are removed under standardconditions of cleavage and deprotection with concentrated ammonia
- A detritylation step is not required since the fluorescein phosphoramiditedoes not contain a DMT-group
- The labeled oligonucleotide is ready for use in most applications after theevaporation of ammonia. Standard procedures can be used if additionalpurification is required.
- Lot-to-lot consistent purity and performance
- Manufactured under a certified ISO 9001 quality system6-Fluorescein Phosphoramidite is configured for ABI Synthesizers.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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