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I0408

Sigma-Aldrich

Invertase Glycoprotein Standard

BioReagent, from Saccharomyces cerevisiae, for proteomics

Synonym(s):

Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase

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About This Item

CAS Number:
9001-57-4
Enzyme Commission number:
3.2.1.26 (BRENDA, IUBMB)
EC Number:
232-615-7
MDL number:
MFCD00131410
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Saccharomyces cerevisiae

Quality Level

200

grade

for proteomics

product line

BioReagent

form

lyophilized powder

mol wt

60 kDa

concentration

≥0.5 mg/vial protein (E1%/280)

storage temp.

2-8°C

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Application

Invertase Glycoprotein Standard has been used:
  • to generate N-linked glycan library
  • as a negative control to study the binding of lectins to high mannose structures
  • for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis
Hilliard M, et al.
MAbs (2017)
Proteomic kinetic analysis of blister fluid and serum in a patient with drug-induced toxic epidermal necrolysis. A comparison with skin immunohistochemistry
Paquet P, et al.
Current Drug Safety (2012)
A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma
Analytical biochemistry (2011)
Ashok K Verma et al.
Plant physiology and biochemistry : PPB, 62, 111-115 (2012-12-05)
Sugarcane is a leading energy crop of the world due to its ability to capitulate high sucrose. To understand the mechanism associated with shoot establishment from the lateral bud of sugarcane setts, at the time of germination, we established shoots
Takashi Tonozuka et al.
Enzyme and microbial technology, 51(6-7), 359-365 (2012-10-09)
Arthrobacter sp. K-1 β-fructofuranosidase (ArFFase), a glycoside hydrolase family 68 enzyme, catalyzes the hydrolysis and transfructosylation of sucrose. ArFFase is useful for producing a sweetener, lactosucrose (4(G)-β-D-galactosylsucrose). The primary structure of ArFFase is homologous to those of levansucrases, although ArFFase
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