H4666
Activin A human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
Activin A Protein, Growth Factor, Human Activin A
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About This Item
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product name
Activin A human, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 25 kDa (non-glycosylated)
packaging
pkg of 5x10 μg
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
storage temp.
−20°C
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Biochem/physiol Actions
Activin-A participates in a diverse array of functions that include; hypothalamic/pituitary/gonadal hormone secretion, insulin secretion; cell growth, differentiation and survival (apoptosis); embryonic patterning and development; wound healing; and inflammation/immune response. It was initially identified as a follicle stimulating hormone/FSH-releasing protein (gonadal hormone), Ling N, et al. (1986) and erythroid differentiation factor (EDF), Schwall R, et al. (1989). The FSH activity is linked to an increase in the population of pituitary gonadotrophs by Katayama T, et al. (1990). In addition to gonadotrophs, activin-A also modifies somatotrophs and lactotrophs, Kitaoka, et al. (1988).
Activin-A stimulates glycogenolysis in isolated rat hepatocytes, Mine T ET. al. (1989) and elevates insulin release from rat pancreatic islets, Verspohl EJ et al. (1993). Activin-A stimulates insulin secretion in rat pancreatic islets, Totsuka Y, et al. (1988).
Follistatin, an activin-binding protein, is a principle regulator of activin activity, de Winter JP, et al. (1996); however during embryogenesis, Cripto is an important noncompetitive activin antagonist that facilitates Nodal signaling, Kelber JA, et al. (2008).
A role for activin-A as a regulator of cell proliferation was recognized by Gonzalez-Manchon C and Vale W. (1989); wherein Act-A inhibited the growth of and induced morphological changes in CHO-KI cells in culture, in a way similar to but less potent than TGF-β.
Activin A is involved with the entire process of embryo development from germ cells thru embryonic development to adult tissues. It stimulates spermatogonial proliferation in germ-Sertoli cell cocultures, Mather JP, et al. (1990) and is a maturation factor for oocytes, Itoh M, et al. (1990). Activin A promotes proliferation of human luteinized preovulation granulose cells (ovarian granulose cells), Rabinovici J, et al. (1990).
Activin-A stimulates glycogenolysis in isolated rat hepatocytes, Mine T ET. al. (1989) and elevates insulin release from rat pancreatic islets, Verspohl EJ et al. (1993). Activin-A stimulates insulin secretion in rat pancreatic islets, Totsuka Y, et al. (1988).
Follistatin, an activin-binding protein, is a principle regulator of activin activity, de Winter JP, et al. (1996); however during embryogenesis, Cripto is an important noncompetitive activin antagonist that facilitates Nodal signaling, Kelber JA, et al. (2008).
A role for activin-A as a regulator of cell proliferation was recognized by Gonzalez-Manchon C and Vale W. (1989); wherein Act-A inhibited the growth of and induced morphological changes in CHO-KI cells in culture, in a way similar to but less potent than TGF-β.
Activin A is involved with the entire process of embryo development from germ cells thru embryonic development to adult tissues. It stimulates spermatogonial proliferation in germ-Sertoli cell cocultures, Mather JP, et al. (1990) and is a maturation factor for oocytes, Itoh M, et al. (1990). Activin A promotes proliferation of human luteinized preovulation granulose cells (ovarian granulose cells), Rabinovici J, et al. (1990).
Analysis Note
The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Sadegh Ghorbani-Dalini et al.
3 Biotech, 10(5), 215-215 (2020-05-02)
The first step in differentiation of pluripotent stem cell toward endoderm-derived cell/organ is differentiation to definitive endoderm (DE) which is the central issue in developmental biology. Based on several evidences, we hypothesized that activin-A optimization as well as replacement of
Yan Li et al.
The Journal of clinical endocrinology and metabolism, 100(11), E1415-E1427 (2015-08-26)
Activin A increases matrix metalloproteinase (MMP) 2 expression and cell invasion in human trophoblasts, but whether the expression of MMP2 is essential for the proinvasive effect of activin A has yet to be determined. Moreover, the identity of the activin
Hannah E J Yong et al.
Pregnancy hypertension, 5(4), 346-353 (2015-11-26)
Activin A, a TGFβ family member, circulates in the maternal blood at increasing concentrations throughout gestation during a healthy pregnancy. The circulating concentration of activin A is further increased in pre-eclampsia (PE), a hypertensive disorder of pregnancy that is marked
Xu Qian et al.
Biomaterials, 35(36), 9581-9590 (2014-09-06)
Well-defined culture conditions are essential for realizing the full potential of human embryonic stem cells (hESCs) in regenerative medicine where large numbers of cells are required. Synthetic polymers such as poly[2-(methacryloyloxy) ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide] (PMEDSAH), offer multiple advantages over
Yutaka Ohsawa et al.
PloS one, 10(7), e0133713-e0133713 (2015-08-01)
Myostatin, a muscle-specific transforming growth factor-β (TGF-β), negatively regulates skeletal muscle mass. The N-terminal prodomain of myostatin noncovalently binds to and suppresses the C-terminal mature domain (ligand) as an inactive circulating complex. However, which region of the myostatin prodomain is
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