G4896
Monoclonal Anti-Gelsolin antibody produced in mouse
clone GS-2C4, ascites fluid
Synonym(s):
Gelsolin Detection Antibody, Mouse Anti-Gelsolin
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About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GS-2C4, monoclonal
contains
15 mM sodium azide
species reactivity
bovine, pig, human, rabbit
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using heat-induced antigen retrieval sections
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using human plasma
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GSN(2934)
General description
Monoclonal Anti-Gelsolin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse. Gelsolin (GSN) is encoded by the gene mapped to human chromosome 9q33.2. GSN is a calcium dependent actin binding protein. It has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called as brevin. GSN is characterized with six domains G1-G6 at N-terminal end, each domain contains Ca2+ binding site.
Specificity
The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.
Immunogen
human plasma gelsolin.
Application
Monoclonal Anti-Gelsolin antibody produced in mouse has been used in immunoprecipitation and immunohistochemical staining.
Biochem/physiol Actions
Gelsolin (GSN) is a potent modulator of actin filament length and gelation. It binds to the plus′ ends of actin monomers and filaments, preventing monomer exchange. GSN plays a vital role in regulation of actin dynamics. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Plasma gelsolin is used as a biochemical marker for acute rheumatic carditis.
Target description
Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200750 Anti-Gelsolin antibody, Mouse Monoclonal
clone GS-2C4, purified from hybridoma cell culture
SAB4200750 Anti-Gelsolin antibody, Mouse Monoclonal
clone GS-2C4, purified from hybridoma cell culture
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Robert Bucki et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4936-4944 (2008-09-20)
The various functions of gelsolin in extracellular compartments are not yet clearly defined but include actin scavenging and antiinflammatory effects. Gelsolin was recently reported to bind endotoxin (LPS) from various Gram-negative bacteria with high affinity. In this study we investigate
Elizabeth S Harris et al.
The Journal of biological chemistry, 279(19), 20076-20087 (2004-03-03)
Formins are a conserved class of proteins expressed in all eukaryotes, with known roles in generating cellular actin-based structures. The mammalian formin, FRLalpha, is enriched in hematopoietic cells and tissues, but its biochemical properties have not been characterized. We show
Naomi Walsh et al.
Journal of proteomics, 71(5), 561-571 (2008-10-14)
Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8
Masaki Ikeda et al.
Journal of the neurological sciences, 252(1), 4-8 (2006-11-14)
Familial amyloid polyneuropathy (FAP: type IV), known as familial amyloidosis of the Finnish type (FAF), is very rare and reported only in a few countries. The gelsolin mutation G654A is most frequent causative gene in FAF family. The clinical phenotype
ATF3 suppresses metastasis of bladder cancer by regulating gelsolin-mediated remodeling of the actin cytoskeleton
Yuan X, et al.
Cancer research, 73(12), 3625-3637 (2013)
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