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Key Documents

KELLY Cell Line human

92110411, human brain, Neuronal

Synonym(s):

NB19 Cells, NB19-RIKEN Cells

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About This Item

UNSPSC Code:
41106514

product name

KELLY Cell Line human, 92110411, neuroblastoma

biological source

human brain

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

N-myc RNA or protein

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human neuroblastoma

Cell Line Description

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Application

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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