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Key Documents

CCRF S-180 II

90011901, mouse unknown/unspecified, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

CCRF S-180 II, 90011901

biological source

mouse unknown/unspecified

growth mode

Adherent

karyotype

2n = 40

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse CFW sarcoma 180

Cell Line Description

Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.

Application

Chemotherapy screening tests

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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