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Idrapril, the prototype of a new class of angiotensin converting enzyme (ACE) inhibitors, competitively inhibits, with nanomolar apparent Ki, the hydrolysis of hippuryl-glycyl-glycine by rabbit lung ACE. Kinetic Ki and kon and koff values were 470 pM, 3.0±1.5×106 M-1sec-1 and 1.4±0.3×10-3 sec-1, respectively. Reference: Biochemical Pharmacology, 45(6), 1358-1362 (1993).
A6778
Angiotensin Converting Enzyme from rabbit lung
≥2.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42010109
NACRES:
NA.32
Pricing and availability is not currently available.
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form
lyophilized powder
Quality Level
specific activity
≥2.0 units/mg protein (modified Warburg-Christian)
shipped in
dry ice
storage temp.
−20°C
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General description
The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site.[1] ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure.[2][3] Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril.[2] Currently, developing inhibitory peptides from natural food sources,[2][3][4] or phenolic compounds from plant sources to inhibit ACE is underway.[5] ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.[1]
Application
Angiotensin converting enzyme from rabbit lung has been used:
- for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)[2]
- to measure the ACE inhibition by litchi pericarp[5] and cooked chicken breast[4] using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC[4] respectively
- in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.[1]
Biochem/physiol Actions
Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.
Quality
May contain traces of sodium chloride.
Unit Definition
One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.
inhibitor
Product No.
Description
Pricing
substrate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Enrichment and biotransformation of phenolic compounds from litchi pericarps with angiotensin I-converting enzyme (ACE) inhibition activity
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Isolation and identification of angiotensin-converting enzyme inhibitory peptides from egg white protein hydrolysates
Liu J, et al.
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The aim of this work is to discover the inhibitory mechanism of tea peptides and to analyse the affinities between the peptides and the angiotensin-converting enzyme (ACE) as well as the stability of the complexes using in vitro and in
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The migration of monocytes into the vessel wall is a critical event leading to the development of atherosclerosis. Monocyte chemoattractant protein-1 (MCP-1) is the main chemotactic factor involved in this phenomenon, and nuclear factor-kappa B (NF-kappa B) is one of
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Are there any other known inhibitors of rabbit lung Angiotensin Converting Enzyme (ACE), Product A6778?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the effect of bleomycin on Angiotensin Converting Enzyme (ACE) activity in vivo?
1 answer-
Bleomycin causes a marked reduction in rabbit lung ACE activity. Reference: Cancer Research, 40, 3621-3626, (1980).
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What is the medical significance of Angiotensin Converting Enzyme (ACE), Product A6778?
1 answer-
Angiotensin-converting enzyme (ACE) produces angiotensin II, a substance that affects the cardiovascular system in numerous ways, including narrowing of blood vessels. This narrowing can cause high blood pressure and cause the heart to work harder. ACE inhibitors treat a variety of conditions, such as high blood pressure, scleroderma and migraines. (Mayo Clinic)
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