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62301

Sigma-Aldrich

Lipase from Aspergillus niger

powder (fine), ~200 U/g

Synonym(s):

Lipase AP6

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

100

form

powder (fine)

specific activity

~200 U/g

mol wt

Mr ~45000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol acetic acid per minute at pH 7.4 and 40 °C (triacetin, Cat. No. 90240 as substrate) 50 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40 °C

Other Notes

Selective acylation and deacylation of furanose and pyranose derivatives; Enantioselective hydrolysis of 2-methyl 3-acetoxy esters; Resolution of the diols of bicycloheptane and bicyclooctane by enzymic hydrolysis; Glyceride synthesis

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W.J. Hennen et al.
The Journal of Organic Chemistry, 53, 4939-4939 (1988)
K. Naemura et al.
Journal of the Chemical Society. Perkin Transactions 1, 2337-2337 (1992)
H. Akita et al.
Tetrahedron Letters, 27, 5241-5241 (1986)
Samantha V Facchetti et al.
Nanomaterials (Basel, Switzerland), 10(12) (2020-12-03)
The large-scale production of plastic and the resulting release of waste is leading to a huge accumulation of micro-sized particles in the environment that could have an impact on not only aquatic organisms but also on humans. Despite the extensive
Alexandra Kotogán et al.
Foods (Basel, Switzerland), 11(12) (2022-06-25)
Hydrolysis of olive, rapeseed, linseed, almond, peanut, grape seed and menhaden oils was performed with commercial lipases of Aspergillus niger, Rhizopus oryzae, Rhizopus niveus, Rhizomucor miehei and Candida rugosa. In chromogenic plate tests, olive, rapeseed, peanut and linseed oils degraded
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