MAB4310X
Anti-CD133 Antibody, clone 13A4, Alexa Fluor™ 488 conjugated
clone 13A4, Chemicon®, from rat
Synonym(s):
Prominin-1, AC133
About This Item
biological source
rat
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
13A4, monoclonal
species reactivity
mouse
should not react with
Drosophila, rat, chicken, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
input
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
chicken ... Prom1(422825)
human ... PROM1(8842)
mouse ... Prom1(19126)
rat ... Prom1(60357)
General description
Specificity
Immunogen
Application
Note: This antibody does not work with paraffin-embedded sections.
EM immunohistochemistry: The subcellular localization of the 13A4 antigen in mouse E9-10 neuroepithelial cells and adult kidney proximal tubule cells was investigated by immunogold electron microscopy. Strong labeling was observed over the kidney brush border membrane, where 13A4 immunoreactivity appeared to be concentrated toward the tips of the microvilli. Remarkably, in neuroepithelial cells, whose apical plasma membrane contains fewer microvilli than the kidney brush border, 13A4 immunoreactivity was associated mostly, if not exclusively, with microvilli and plasma membrane protrusions, and was not detected in the planar areas of the apical plasma membrane. Because of this preferential localization, the 13A4 antigen was referred to as ′′prominin′′ (from the Latin word ′′prominere,′′ to stand out, to be prominent) (Weigmann et al., 1997).
Western blotting: 1-5 μg/mL in 0.3% Tween in PBS. Sample preparation: Standard Laemmli (boiled in 2% SDS, 100mM DTT or 5% beta-mercaptoethanol, 60mM Tris-HCL pH 6.8). Preferred Gel percentage: 7.5%. Suggested Blocking Buffer: 3-5% Milk, 0.3% Tween in PBS. Incubation time: 1 hour at room temperature or overnight at 4°C. Recommended control extracts: Positive: Kidney membrane; Negative: liver membranes.
Immunoprecipitation: 10-25 μg/mL. Suggested tissue/cell lysis buffer: RIPA bufferFinal reaction volume: 500-1000 μL. Final total protein concentration in reaction mix: 0.5-3 mg/mL. Incubation times: overnight at 4°C. Capture agent used: Protein G Sepharose™ or rabbit anti-rat antibody/protein A Sepharose. Expected sizes on immunoblots (in kDa): 115 kDa (mature form) or 105 kDa (precursor form).
FACS Analysis: Suggested dilution/number of cells: 0.25-1 μg/ million cells. Fixation/Permeabilization used: BD FACS lysis Solution (1-1.5% formaldehyde) (BD and FACS are trademarks of Becton, Dickinson and Company) No permeabilization. Recommended controls: Hematopoietic stem cells.
Optimal working dilutions must be determined by the end user.
Stem Cell Research
Neural Stem Cells
Hematopoietic Stem Cells
Physical form
Storage and Stability
Analysis Note
Human embryonic stem cell lysate, Caco 2 (Human colonic carcinoma cell line) whole cell lysate
Other Notes
Legal Information
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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