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ABS21

Sigma-Aldrich

Anti-Surfactant Protein B Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Pulmonary surfactant-associated protein B, 18 kDa pulmonary-surfactant protein, Pulmonary surfactant-associated proteolipid SPL(Phe), SP-B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SFTPB(6439)

General description

Surfactant Protein B (SP-B) is mostly composed of phospholipids, but also incorporates essential proteins. It is necessary for pulmonary surfactant function and the alveolar type 2 cell phenotype. SP-B deficiency has been known to cause congenital pulmonary alveolar proteinosis. SP-B is thought to protect lungs against endotoxin-induced inflammation. SP-B could be used as a clinical marker in chronic heart failure.

Specificity

This antibody recognizes Surfactant Protein B at the Saposin B-type 2 domain.

Immunogen

Epitope: Saposin B-type 2 domain
GST-tagged recombinant protein corresponding to human Surfactant Protein B at the Saposin B-type 2 domain.

Application

Immunohistochemistry Analysis: 1:200 dilution from a representative lot detected Surfactant protein B in clara cells in normal rat lung tissue.
Research Category
Signaling
Research Sub Category
Lipid Signaling
This Anti-Surfactant Protein B Antibody is validated for use in WB, IH(P) for the detection of Surfactant Protein B.

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1:5,000 dilution of this antibody detected Surfactant Protein B on 10 µg of human lung tissue lysate.

Target description

~16 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qingmiao Zhang et al.
FEBS open bio, 8(10), 1594-1604 (2018-10-20)
Hyperglycemia during pregnancy is associated with fetal lung development disorders and surfactant protein (SP) deficiency. Here, we examined the role of FOXA2 and Akt signaling in fetal lung development during diabetic pregnancy. Sprague-Dawley rats were injected with streptozocin (STZ) during

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