2752-M

BrdU Cell Proliferation Kit

• Synonym(s): Bromodeoxyuridine Assay, non isotopic assay
• UNSPSC Code: 12161503
• NACRES: NA.76

Properties

• species reactivity: guinea pig, rabbit, pig, mouse, horse, rat, canine, nonhuman primates, hamster, human
• Quality Level: 100
• packaging: pkg of 1000 assay(s)
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable
• detection method: colorimetric

Description

General description

The CHEMICON^® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.

Test Principle

Within CHEMICON^®′s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.

For Research Use Only. Not for use in diagnostic procedures.

Application

The BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.

Packaging

1000 assays

Components

• BrdU Reagent: (Part No. 2752a) One 100 μL vial of a 500X solution of BrdU.
• Fixing Solution: (Part No. 2752b) One 200 mL bottle.
• Prediluted BrdU Detection Antibody: (Part No. 2752c) One 100 mL vial of anti-BrdU in solution.
• Stop Solution: (Part No. 2752d): One 100 mL bottle of 2.5N sulfuric acid solution.
• Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2752e) One 60 μL vial of a 2000X solution.
• Conjugate Diluent: (Part No. 2752f) One 100 mL bottle.
• Substrate: (Part No. 2752g) One 100 mL bottle, ready to use TMB.
• 50X Plate Wash Concentrate: (Part No. 2752h) One 125 mL bottle of buffered Tris with surfactant.

Storage and Stability

Maintain the unopened kit at 2-8°C until the expiration date indicated on the label.

Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids